Figure 2
From: Purification and Characterization of Human Adrenomedullin Binding Protein-1
(A) SDS-PAGE analysis of purified human AMBP-1. Lane 1, commercial AMBP-1 (reducing); lane 2, purified AMBP-1 (reducing); lane 3, marker (PageRuler Prestained Protein Ladder; Crystalgen Inc., Plainview, NY, USA); lane 4, commercial AMBP-1 (nonreducing); lane 5, purified AMBP-1 (nonreducing); lane 6, human serum (nonreducing); lane 7, marker. (B) Western blot analysis. Lane 1, commercial AMBP-1 (nonreducing); lane 2, purified AMBP-1 (nonreducing); lane 3, marker (MagicMark XP Western Protein Standard; Invitrogen, Carlsbad, CA, USA). (C) Binding assay. Lane 1, commercial AMBP-1; lane 2, purified AMBP-1; lane 3, control protein.