Figure 3

Purified human AMBP-1 associated with AM suppressed LPS-induced TNF release. The monolayer of rat peritoneal macrophage (A), murine-like macrophage RAW 264.7 cells (B), and Kupffer cells (C) was incubated with or without LPS (10 ng/mL) in the absence or presence of human AM (200 nM)/purified AMBP-1 (100 nM), purified or commercially available. The levels of TNF-α in the culture medium were determined by ELISA and expressed as mean ± SEM of three or five independent experiments (in duplicate) and compared by one-way ANOVA and Student-Newman-Keuls method. *P < 0.05 compared with None; #P < 0.05 compared with LPS alone.