Figure 4

Purified human AMBP-1 associated with human AM suppressed LPS-induced IL-6 (A) release, but had no effect on MCP-1 (B) in rat peritoneal macrophages. Monolayer of cultured rat peritoneal macrophages was incubated with or without LPS (10 ng/mL) in the absence or presence of human AM (200 nM)/AMBP-1 (100 nM), purified or commercially available. The levels of IL-6 in the culture medium were determined by ELISA. The levels of MCP-1 were determined by an immunoassay kit. The results were expressed as mean ± SEM of three or six independent experiments (in duplicate) and compared by one-way ANOVA and Student-Newman-Keuls method. *P < 0.05 compared with None; #P < 0.05 compared with LPS alone.