Figure 5

Stability of purified human AMBP-1. Monolayer of rat peritoneal macrophages was incubated with or without LPS (10 ng/mL) in the absence or presence of human AM (200 nM)/AMBP-1 (100 nM), purified or commercially available. The isolated and purified human AMBP-1 was well preserved when stored at 45°C for 5 d in solution or 100°C for 1 h in powder. Purified human AMBP-1 solution stored at -20°C was used as a positive control. The levels of TNF in the culture medium were determined by ELISA and expressed as mean ± SEM of three independent experiments (in duplicate) and compared by one-way ANOVA and Student-Newman-Keuls method. *P < 0.05 compared with None; #P < 0.05 compared with LPS alone.