Figure 3

(A) Representative spectra of Fischer rats (control: n = 4; L-NAME: n = 4) obtained on CM10 ProteinChip Arrays. (B) Relative intensity of 22.4 kDa peak in each group of rats. The secretion of this marker was only observed in the hypertensive Fischer rats, and losartan treatment significantly decreased its release into the culture medium. *P < 0.05 compared with control, one-way ANOVA. **P < 0.01. ***P < 0.001 compared with control rats. §P < 0.05 compared with L-NAME, one-way ANOVA. (C) Coomassie-stained 12% Bis-Tris gel after purification of the secreted 22.4 kDa protein by anion exchange and reverse phase chromatography (marker of interest ≈ 22 kDa is shown by an arrow) and reprofiling of the gel-extracted protein using SELDI-TOF MS. (D) Amino acid sequence of SM22 a, 5 peptides identified by tandem mass spectrometry are underlined (see Table 4).