Figure 4

Secretion of ubiquitin by hypertensive rats. (A) Representative spectra of Fischer rats (control: n = 4; L-NAME n = 4) obtained on CM10 ProteinChip arrays. (B) Relative intensity of the 8.5 kDa peak in each group of rats. The secretion of this marker was influenced by both strain and L-NAME with an interaction. An increase was observed in reno-vascular rats while losartan diminished the intensity in Fischer L-NAME rats. *P < 0.05 compared with control, one-way ANOVA. ***P < 0.001 compared with control rats. §P < 0.05 compared with L-NAME, one-way ANOVA. §§§P < 0.001 compared with L-NAME rats. (C) Coomassie-stained 16% Tricine gel after purification by anion exchange and reverse phase chromatography (marker of interest ≈ 8.5kDa is shown by arrow) and the spectrum obtained on NP20 array for the band of interest. (D) Amino acid sequence of ubiquitin. Six peptides identified by tandem mass spectrometry are underlined (see Table 4).