Figure 5

Secretion of the C-terminal fragment of filamin A. (A) Representative spectra of Fischer rats (control: n = 4; L-NAME n = 4) obtained on IMAC30 ProteinChip arrays. (B) Relative intensity of the 10.7 kDa peak in each group of rats. The release of this marker was influenced by both strain and L-NAME and there was a significant interaction. An increase was observed in renovascular rats while losartan diminished the intensity in Fischer L-NAME rats. *P < 0.05 compared with control, one-way ANOVA. ***P < 0.001 compared with control rats. §§P < 0.01 compared with L-NAME, one-way ANOVA. (C) Coomassiestained 16% Tricine gel after purification by anion exchange and reverse phase chromatography (marker of interest ≈ 10.7 kDa is shown by arrow) and the spectrum obtained on NP20 array for the band of interest.(D) This protein was digested with trypsin and analyzed by tandem mass spectrometry. The amino acid sequence was obtained for three peptides (bold underlined, see Table 4).