Figure 6

Secretion of thymosin β4 by hypertensive rats. (A) Representative spectra of Fischer rats (control: n = 4; L-NAME n = 4) obtained on CM10 ProteinChip arrays. (B) Relative intensity of the 4.9 kDa peak in each group of rats. The release of this biomarker was influenced by both strain and L-NAME, and there was a significant interaction. An increase was observed in renovascular rats, while losartan diminished the intensity in Fischer L-NAME rats. *P < 0.05 compared with control, one-way ANOVA. ***P < 0.001 compared with control rats. §§§P < 0.001 compared with L-NAME, one-way ANOVA. (C) This bio-marker was analyzed by tandem mass spectrometry after chromatographic purification (reverse phase column) followed by a direct AspN digestion (without separation on gel). The amino acid sequence of the peptide analyzed by MS/MS is presented in Table 3.