Figure 7
Detection of ubiquitin in conditioned medium and aortic wall. A) Representative blots of secreted proteins from two rats of each group. Proteins (loading normalized to aortic wet weight) were electrophoresed on an 18% acrylamide gel and transferred onto nitrocellulose membranes. Membranes then were probed with a monoclonal antibody to ubiquitin (1:250). B) Ubiquitin immunostaining in aortas of L-NAME and control Fischer rats.