Figure 8

Detection of SM22α in conditioned medium and in plasma. (A) Representative blots of secreted proteins from two rats of each group. Proteins (loading standardized by aortic wet weight) were electrophoresed on a 12.5% acrylamide gel and transferred onto nitrocellulose membranes. Membranes were then probed with a monoclonal antibody to SM22 α (1:1,000). (B) Representative blots of albumin-depleted plasma (15 µL, n = 2 for each group). Western blots were performed using the same conditions as those used for the conditioned medium.

*P < 0.05 compared with control rats.