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Figure 4 | Molecular Medicine

Figure 4

From: Pathogenic Autoantibodies in Systemic Lupus Erythematosus Are Derived from Both Self-Reactive and Non-Self-Reactive B Cells

Figure 4

The effects of individual point mutations on the binding of antibodies to dsDNA. (A) Back-mutated variants. Both germline and mutated amino acid sequences are shown. An underline means the mutated residue was reverted to the germline sequence. # denotes the original mutated antibody and f denotes the unmutated counterpart. (B) A9 and its variants were tested by ELISA for reactivity with dsDNA at 5 jig/mL and three subsequent 1:4 dilutions. Clone 53 from MC Nussenzweig’s laboratory was used as a negative control (dotted line).

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Molecular Medicine

ISSN: 1528-3658