Figure 1

(A) Effect of PBS, fibroblasts, and NSC i.v. treatments on locomotor activity after SCI in open field. Locomotor recovery was evaluated over a 3-month period, using the nine-point BMS scale. Values represent mean ± SEM. We analyzed the statistical differences with the ANOVA test followed by Bonferroni’s post-test method. ▼ Laminectomized mice; ● NSC-treated animals; ▽ Fibroblast-treated mice; ◯ PBS-treated mice. Significance symbols: $, Laminectomized mice versus all other groups; ′, NSC-treated mice versus fibroblast-treated mice; °, NSC-treated mice versus PBS-treated mice; *, Fibroblast-treated mice versus PBS-treated mice. $$$,°°°,′′′,***P < 0.001; $$,°°,′′,**P < 0.01; $,°,’,*P < 0.05. BMS, Basso Mouse Scale (nine animals per group were used in this analysis). (B) Effect of NSC administration via i.v. route or intraspinal injection on open field locomotor activity after SCI. Hind limb recovery of function was evaluated over a 2-month period using the nine-point BMS scale. Values represent average ± SEM. We determined the statistical differences by means of an ANOVA test followed by Bonferroni’s post-test. For this experiment the number of animals was six per group. ● Animals treated with NSCs by i.v. injection; ◯ Animals treated with NSCs by intraspinal injection; ▽ Animals treated with PBS by intraspinal injection. °, NSC i.v.-treated mice versus NSC intraspinal-transplanted mice; *, NSC intraspinal-transplanted mice versus PBS intraspinal-treated mice; °°°,***p < 0.001; °°,**P < 0.01; °,*P < 0.05.