Figure 4

Immunolocalization of the Nestin, β-Tubulin III, and SSEA1-positive cells at the site of the lesion 3 wks after the contusion. Serial cryostat transversal section 10 µm thick. (A) GFP-positive cell (green) localization 3–4 wks after i.v. transplantation in a transversal section of a spinal cord; (B) Nestin staining (red); (C) β-Tubulin III staining (red); (D) SSEA1 staining (red); (E-G) Green fluorescence (in green) (indicated by the arrows) of the section corresponding respectively to B, C, and D; (H-L) Merge of the section B-E, C-F, and D-G (the arrows indicated the double positive cells). Scale bars 10 µm are shown only in A and B. The nuclei (4,6-diamidine-2-phenylindole dihydrochloride (DAPI)-positive) are indicated in blue; many GFP-positive cells have the nuclei labeled with DAPI.