Figure 6

Confocal analysis of the GFP-NSCs recovered 1 wk after transplantation. A, B, C, D show GFP-NSCs grown in culture as positive control (A) and the antibody staining to GFP (using Alexa 546 labeled secondary antibody) labels all the cells (B). Merge in D, all cells are positive. E, F, G, H show GFP-NSCs grown in culture as positive control (H) and no antibody to GFP labels was added (F) (negative control for the antibody staining). Merge in H all cells are, however, green positive, but not stained for the antibody. I, J, K, L show wild-type NSC obtained from a non-GFP-wild type mouse presented as negative control. Negative in fluorescence for GFP and no labeling with antibody to GFP (I and J). The nuclei show the presence of stem cells (K and L), but labeling is lacking. M, N, O, P show GFP-NSCs recovered from lesion site 1 wk after i.v. administration (M) and positive to anti-GFP-antibody (N); the nuclei are more numerous (O), indicating presence of non-GFP-NSCs from the cord. Merge (P) indicates that 36% of these stem cells are transplanted cells. C, G, K, and O show DAPI staining of the nuclei. Scale bar 50 µm in D is representative for all the pictures.