Figure 3

Decreased insulin receptor substrate-1 (IRS-1) and insulin receptor (IR) tyrosine phosphorylation in skeletal muscle following trauma and hemorrhage. Rats were subjected to trauma alone (T) or trauma and hemorrhage (TH) as described in Figure 1 except tissue lysates were subjected to Western blotting with antibodies specific for phospho-IRS-1 tyrosine 612 (P-IRS-1 Y612), total ERK1/2 (loading control), phospho-IR tyrosine 972 (P-IR Y972) and total IR antibodies. (A) Representative Western blots are presented and (B) autoradiographs were quantified by scanning densitometry; the data are presented as mean ± SEM fold change of P-IRS-1 (Y612) by insulin of three rats (n = 3) in each group. (C) Representative Western blots are presented and (D) autoradiographs were quantified by scanning densitometry; the data are presented as mean ± SEM fold change of P-IR (Y972) by insulin (n = 3 in each group). T0′ with no insulin treatment was arbitrarily set to 1. #P < 0.05 compared with T0′; *P < 0.05 compared with T only group at the same time point 0.