Figure 7

The recovery of skeletal muscle insulin signaling via phospho-Akt (P-Akt) following trauma and hemorrhage. Rats were subjected to trauma alone (T), or trauma and hemorrhage (TH). At 5h or 24h following resuscitation, either saline (−) or 5U insulin (+) was injected via the portal vein. Tricep lysates were subjected to Western blotting with antibodies specific for P-Akt serine 473 (S473) or total Akt (not shown). Representative Western blots of at least three separate experiments from each time point, TH5h and TH24h, are presented.