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Table 2 Antigenic reactivities of CLL mAbs

From: Chronic Lymphocytic Leukemia Cells Recognize Conserved Epitopes Associated with Apoptosis and Oxidation

     Reactivity with
CLL mAb Subse†a IGHV gene Mutation statusa Healthy HEp-2 cellsb Apoptotic RAMOS B cellsc Apoptotic Jurkat T cellsc MDA-BSAd POVPC-BSAd HNE-BSAd PC-BSAd Oxidized LDLd
068 6 1–69 U-CLL + + + +    ++  
258 6 1–69 U-CLL + + +
014 9 1–69 U-CLL + + + ++++ +++ + + +
246 9 1–69 U-CLL + +++ ++ + +
355 9 1–69 U-CLL + + + ++++ ++ +
358 NA 1–69 M-CLL +
154 1 1–18 M-CLL + + + +
340 1 1–2 U-CLL + + + ++ +
360 1 1–3 U-CLL ++ ++ + ++ +
270 1 1–2 U-CLL + + + ++++ + +
DO13 28 1–2 U-CLL ++ +++ + ++++ + + ++ ++
376 NA 1–24 U-CLL +
415 NA 1–3 U-CLL + + + + +
366 9 3–21 U-CLL + ++ + ++ + + +
282 2 3–21 M-CLL +
412 2 3–21 M-CLL +
562 NA 3–21 U-CLL +
169 NA 3–33 M-CLL +
141 NA 4–34 U-CLL + + +
DO8 NA 4–34 U-CLL + + + + + +
183 4 4–34 M-CLL +
240 4 4–34 M-CLL +
342 4 4–34 M-CLL + + + + +
114 8 4–39 U-CLL ++++ + + +++ + + +
657 8 4–39 U-CLL ++++ ++ ++ ++++ + + ++
845 8 4–39 U-CLL ++++ + + +++ + ++ +
260 NA 4–b U-CLL + + ++ + +
255 NA 4–59 M-CLL ++ + +
  1. aStereotypic subset and mutation status as in Table 1.
  2. bBinding to live HEp-2 cells (subsequently fixed and permeabilized) in arbitrary units based on immunofluorescence intensity.
  3. cBinding to apoptotic RAMOS and Jurkat cells by flow cytometry as determined by percent positive events: − = < 2%; + = 2%−5%; + + = 5%−10%; + + + = 10%−15%; + + + + = 15%−20%.
  4. dBinding to antigens in ELISA based on signal-to-background ratio: − = < 2; + = 25; + + = 5–10; + + + = 10–15; + + + + = > 15.