Figure 1

Expression of Bcl-2 mRNA and protein. (A) Microarray analysis of Bcl-2. Probe sets for Bcl-2 found to be significantly different at initial gene expression screening by microarray. The first column is the Affymetrix chip version, the second column is the fold change of B-CLL samples compared with samples from normal subjects (n = 59), and the third column is the t test value (one-tailed, type 2). (B) RT-QPCR verification of Bcl-2 levels. Graphic representation of data for the first round of RT-QPCR verification. Eighteen paired samples from B-CLL patients and normal individuals were analyzed for fold change differences. Control data were normalized to one and the delta delta CT method of analysis was employed. (C) Summary chart of the Bcl-2 RNA message. Numbers represent the mean log fold change of Bcl-2 mRNA as measured by RT-QPCR for the samples shown in Figure 1B. (D)Western blot analysis of Bcl-2 protein levels. Lysates of B cells from five B-CLL patients on the left and three healthy subjects matched for age on the right were separated by SDS-PAGE electrophoresis and probed with a Bcl-2-specific antibody. The presence of very similar amounts of β actin in each lane indicates similar protein loading for each lane.