Table 1 Gene-specific primer sequences, LightCycler run conditions, and PCR efficiencies
From: Transcriptional Control of Complement Activation in an Exercise Model of Chronic Fatigue Syndrome
Gene name (symbol) | GenBank accession # |  | Primer sequence (5′→3′)a | Product size (bp) | Annealing/acquisition temperature | PCR efficiency |
|---|---|---|---|---|---|---|
Complement component 1, q subcomponent A chain (C1QA) | NM_015991 | FW: | GGCAGCCCAGGAAACATCAAGGAC | Â | Â | Â |
| Â | Â | RV: | AATCGGCCGGAGTGGTTCTGGT | 143 | 65/87 | 1.96 |
Complement component 1, s subcomponent (C1S) | NM_001734 | FW: | CATGGATGGGGACCTGGGACTGA | Â | Â | Â |
| Â | Â | RV: | GCCTCTGCATCTGCTGTGGGTTTC | 150 | 65/84 | 1.99 |
Complement component 4 (C4) | NM_007293 | FW: | GGGGCCCCACGTCCTGCTGTAT | Â | Â | Â |
| Â | Â | RV: | CTGCGCTCGGGGTTGTAGTAGTCG | 141 | 65/89 | 2.00 |
Mannose-binding lectin 2 (MBL2) | NM_000242 | FW: | ACAAACTGGAACGAGGGTGAA | Â | Â | Â |
| Â | Â | RV: | CAGATGGGAGGTGGAGCAG | 151 | 65/85 | 2.00 |
Mannan-binding lectin serine protease 2 (MASP2) | NM_006610 | FW: | TATGAAAAGCCACCCTATCCA | Â | Â | Â |
| Â | Â | RV: | TGCCCCTCCGCTGTCAC | 105 | 60/84 | 1.99 |
Ficolin 1 (FCN1) | NM_002003 | FW: | CGCCGACTGTCATGCTTCAAACCT | Â | Â | Â |
| Â | Â | RV: | GTACCCCTTCGCCGCACTCCAG | 97 | 65/82 | 2.05 |
Ficolin 2 (FCN2) | NM_004108 | FW: | GAACCAGCGAGCTCCGTGTAGACC | Â | Â | Â |
| Â | Â | RV: | GAAGGCCCCCAGGACCAGATTGT | 125 | 65/83 | 1.95 |
Complement component 1 inhibitor (SERPING1) | NM_000062 | FW: | ACAGCAGCAGCCCCAGAGTCCTAA | Â | Â | Â |
| Â | Â | RV: | AGCCGGCTGATCTTGTTGTTGGTG | 100 | 65/84 | 1.97 |
Interferon γ (IFN-γ) | NM_000619 | FW: | TTGGGTTCTCTTGGCTGTTACTG |  |  |  |
| Â | Â | RV: | TGGCTCTGCATTATTTTTCTGTCA | 167 | 65/78 | 1.99 |
Peptidylpropyl isomerase B (PPIB) | M60857 | FW: | AAGGGGCCCAAAGTCACCGTCAAG | Â | Â | Â |
| Â | Â | RV: | GGGGAAGCGCTCACCGTAGATGC | 261 | 65/82 | 1.95 |
Phosphoglycerate kinase 1 (PGK1) | NM_000291 | FW: | GCAGATTGTGTGGAATGGTC | Â | Â | Â |
| Â | Â | RV: | CCCTAGAAGTGGCTTTCACC | 101 | 65/81 | 1.98 |
Ribosomal protein, large, PO (RPLPO) | NM_001002 | FW: | AAACTCTGCATTCTCGCTTCCTG | Â | Â | Â |
| Â | Â | RV: | GACTCGTTTGTACCCGTTGATGAT | 119 | 65/83 | 1.98 |
Eukaryotic elongation factor 1 (EEF1A1) | NM_001402 | FW: | TGCGGTGGGTGTCATCAAA | Â | Â | Â |
| Â | Â | RV: | AAGAGTGGGGTGGCAGGTATTG | 123 | 65/84 | 2.00 |
Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) | U91966 | FW: | ATTAGATGGCCAAGAGTAGATGAA | Â | Â | Â |
| Â | Â | RV: | AAAAAGATTGAGCCGAGTGC | 83 | 57/73 | 1.99 |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | X01677 | FW: | ACCACAGTCCATGCCATCAC | Â | Â | Â |
| Â | Â | RV: | TCCACCACCCTGTTGCTGTA | 450 | 58/86 | 1.95 |