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Table 3 Over- or underexpression of complement genes as defined by ≥50% of CFS subjects who showed ≥ two-fold expression changes in response to an exercise challenge compared with the median of the control group. a

From: Transcriptional Control of Complement Activation in an Exercise Model of Chronic Fatigue Syndrome

 

Normalization method

 

PPIB b

GM

rbcL

Gene

T0

T1

T2

T0

T1

T2

T0

T1

T2

C1QA

—

62.50

57.14

—

—

—

50.00

—

—

C1S

—

—

—

—

62.50

—

—

—

—

C4

—

75.00

—

—

62.50

—

—

62.50

—

MASP2

62.50

62.50

57.14

—

50.00

—

50.00

62.50

—

FCN1

—

62.50

—

—

50.00

—

—

62.50

—

FCN2

50.00

50.00

—

—

—

—

—

—

—

SERPING1

—

—

—

50.00

—

—

—

—

—

IFN-γ

62.50

—

—

50.00

50.00

57.14

—

—

—

  1. aOnly values for ≥50% of CFS subjects that showed ≥ two-fold differential expression compared with the median of the control group are shown. Cells with dashes indicate no differential expression.Values indicating downregulation are underlined. Because of a missing sample, values under T2 (in italics) indicate percentage with a denominator of seven CFS subjects as opposed to T0 and T1 percentage values with a denominator of eight CFS subjects.
  2. bPPIB = normalization by the internal reference gene PPIB; GM = normalization by geometric mean; rbcL = normalization by the external reference gene rbcL. T0 = preexercise baseline; T1 = 1 h postexercise; T2 = 6 h postexercise. Numbers in lightly shaded cells indicate differential expression by MASP2 and IFN-γ at T0 by at least two methods of normalization. Numbers in darkly shaded cells indicate differential expression in C4, MASP2, and FCN1 at T1 by all three normalization methods.