Figure 3
From: Rational Optimization of a Bispecific Ligand Trap Targeting EGF Receptor Family Ligands
EGFRT15S/Fc and HER3Y246A/Fc have improved affinity for their cognate ligands. Saturation binding of 125I-ligands was performed in anti-Fc–coated 96-well plates with the indicated HER/Fc proteins immobilized on the surface. Binding data were plotted as fractions of maximal 125I-ligands bound. Shown are 125I-EGF binding (A), 125I-TGF-α binding (B), 125I-HB-EGF binding (C), and 125I-NRG1-β binding (D). Results are means ± SEM of triplicate wells.