Figure 4
From: Rational Optimization of a Bispecific Ligand Trap Targeting EGF Receptor Family Ligands
RB242 has restored high-affinity for EGFR ligands. Ligand binding was performed in anti-Fc–coated 96-well plates using the optimized ligand binding conditions as detailed in Methods. Saturation binding of Eu-EGF and Eu-NRG1-β (A,B). Displacement of Eu-EGF with unlabeled TGF-α or HB-EGF (C,D). Results were representatives of three independent experiments, and were normalized to fractions of receptors bound with ligands (with the largest mean value in each dataset defined as 1).