Figure 1
From: CD38 and CD157 Ectoenzymes Mark Cell Subsets in the Human Corneal Limbus
Immunodetection of the CD38 and CD157 molecules expressed by human corneal cells. Isolated human corneal cells were stained with anti-CD38 (AT13/5, IgG1), anti-CD157 (SY/11B5, IgG1) mAbs, or with an isotype-matched irrelevant control mAb. (A) Staining of CD38 and CD157 with specific mAbs in surface-scraped or cultured epithelial cells, with corresponding control and bright field images. (B) Staining of CD38 and CD157 in enzyme-isolated or cultured stromal cells, with corresponding control and bright field images. (C) Fluorescent images acquired using confocal microscopy, showing the staining of CD38 and CD157 in surface-scraped corneal epithelial cells and corresponding fields seen with interference contrast. The molecules are localized at the membrane levels of the corneal epithelial cells.