Figure 3

Immunostaining of the CD38 and CD157 molecules in cryostat sections of human cornea. Sections were stained with anti-CD38 (AT13/5, IgG₁), anti-CD157 (SY/11B5, IgG₁) mAbs, and with an isotype-matched irrelevant control mAb. (A) CD38⁺ cells are visible in the superficial and suprabasal limbal epithelium. No signals are detected in the basal limbal epithelium or in the stroma. (B) CD157⁺ cells are apparent at the basal limbus and in the stroma. (C) The CD157 signal fade away to the corneal-limbal interface epithelium, above the Bowman’s capsule (arrow). Inset: corresponding bright field images of limbus, corneal-limbal interface, and corneal epithelium (A-C). The junction between limbal and corneal epithelium was determined by the termination of the Bowman’s capsule (arrow). Scale bars: (A) 50 µm; (B,C) 75 µm.