Figure 4

Immunostaining of CD157, p63, and CK19 in cryostat sections of human cornea. Sections were stained with anti-CD157 (SY/11B5, IgG₁), anti-CK19, and anti-p63 mAbs. Upper images: Staining of CD157 clusters (A) and nuclear p63 (B) in the basal limbal epithelium and the stroma underlying the palisades of Vogt (A-D). Merged confocal image (C) shows that selected CD157⁺ cells are p63⁺ (arrows). The corresponding bright field image of the limbus is shown (D). Lower images: Staining of CD157 (E) and CK19 (F) in the basal corneal-limbal epithelium (E-H). The merged confocal image (G) shows that CK19⁺ cells are CD157⁺. The corresponding bright field image of the corneal-limbal epithelium is shown (H). Scale bars: 15 µm.