Figure 4

Validation of microarray results obtained using QPCR to analyze expression levels of EDF1, TFPI, CDH4, and TNFAIP3 genes. Total RNA from the same RNA samples that were subjected to microarray study were used in the QPCR validation reactions. Values were expressed as mRNA gene expression level (for each specific gene)/housekeeping gene fold-change expression compared with the reference sample. The gene expression of the study genes was identified as significant differentially expressed when comparing different group of tissues samples. Specifically, EDF1was identified as differentially expressed when HCV cirrhotic tissues from patients with HCC (nontumor samples) (n = 17) were compared with HCV cirrhotic tissues from patients without HCC (n = 41) (A). TFPI was differentially expressed in HCV-HCC samples (n = 47) when compared with HCV cirrhotic tissues from patients with HCC (n = 17) (B). CDH4 and TNFAIP3 were identified as differentially expressed when HCV-HCC samples (n = 47) were compared with HCV cirrhotic tissues (n = 41) (C).