Figure 2
From: Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin
(A) Separated mitochondria were identified by Janus green B staining. The isolated mitochondria were stained as bluish green round particles. (B) Validation of Raji cell mitochondrial purity by Western blotting. An equal amount of proteins (20 µg) were loaded onto a 10% SDS-PAGE with indicated antibodies against marker proteins from mitochondria (mito) and nuclei and lysosomes (nuclei/lysosome). Antibodies against COXIV were used as a marker specific for mitochondria. Antibodies against PCNA and cathepsin D were used as markers for nuclear and lysosome proteins, respectively. The results showed that COXIV was specifically detected in the purified mitochondrial fraction, and this fraction lacked any detectable contamination by abundant nuclear and lysosome proteins such as PCNA and cathepsin D.