Table 1 Identified differentially expressed mitochondrial proteins of Raji cells treated with ADR.
From: Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin
Up- or downregulated proteins | Function group | Protein name (gene name) | Accession no.a | Molecular mass | pl | GRAVY | Average ratio |
|---|---|---|---|---|---|---|---|
Upregulated | Â | Â | Â | Â | Â | Â | Â |
| Â | OXPHOS | Â | Â | Â | Â | Â | Â |
|  |  | ATP synthase d chain, mitochondrial (ATPQ, ATP5H) | O75947 | 18491.21 | 5.21 | −0.554 | 1.93 |
| Â | Cell cycle | Â | Â | Â | Â | Â | Â |
| Â | Â | Prohibitin (PHB) | P35232 | 29804.1 | 5.57 | 0.024 | 2.53 |
| Â | Transporters and channels | Â | Â | Â | Â | Â | Â |
|  |  | Heat shock 70 kDa protein 9 precursor (HSPA9) | P38646 | 73680.5 | 5.87 | −0.4 | 2.29 |
Downregulated | Â | Â | Â | Â | Â | Â | Â |
| Â | OXPHOS | Â | Â | Â | Â | Â | Â |
|  |  | LOC644189 Similar to Acyl-coenzyme A thioesterase 2 (ACOT2) | P49753 | 53237.52 | 8.82 | −0.169 | −3.52 |
|  |  | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | P04406 | 36053.21 | 8.57 | −0.108 | −3.52 |
|  |  | Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (Precursor) (HADH) | Q16836 | 34277.5 | 8.88 | −0.123 | −3.52 |
| Â | Cell cycle | Â | Â | Â | Â | Â | Â |
|  |  | HRAS-like suppressor 2 (HRASLS2) | Q9NWW9 | 18750.4 | 5.37 | −0.029 | −2.26 |
|  |  | Kinetochore protein Hec1 (NDC80) | O14777 | 73912.67 | 5.48 | −0.749 | −3.52 |
| Â | Transporters and channels | Â | Â | Â | Â | Â | Â |
|  |  | Geranylgeranyl transferase type-1 subunit β (PGGT1B) | P53609 | 42396.3 | 6.37 | −0.265 | −3.05 |
|  |  | Isoform 1 of Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 (NDST2) | P52849 | 100874.65 | 8.81 | −0.261 | −3.05 |
|  |  | Hemoglobin subunit epsilon (HBE1) | P02100 | 16319.93 | 8.69 | 0.027 | −3.52 |
|  |  | HBA1 Hemoglobin subunit α (HBA2) | P69905 | 15257.55 | 8.72 | 0.048 | −3.52 |
| Â | DNA repair | Â | Â | Â | Â | Â | Â |
|  |  | DNA repair protein RAD52 homolog (RAD52) | P43351 | 24539.89 | 8.43 | −0.735 | −3.05 |
|  |  | DNA mismatch repair protein Msh3 (MSH3) | P20585 | 127455.94 | 8.30 | −0.327 | −2.26 |
|  |  | Similar to Fanconi anemia group F protein (FANCF) | Q9NPI8 | 42254.4 | 9.11 | −0.307 | −2.45 |
| Â | Protein synthesis and degradation | Â | Â | Â | Â | Â | Â |
|  |  | Elongation factor Tu, mitochondrial (Precursor) (TUFM) | P49411 | 49541.54 | 7.26 | −0.122 | −3.05 |
|  |  | KIAA0415 gene product (KIAA0415) | A4D1Z4 | 164676.62 | 8.76 | −0.080 | −3.05 |
|  |  | Isoform 4 of Mitochondrial ATP-binding cassette sub-family B member 6 (ABCB6) | Q9NP58 | 93885.56 | 8.75 | 0.149 | −2.26 |
|  |  | Isoform 2 of Uncharacterized protein C19orf60 (C19orf60) | Q96EN9 | 22598.74 | 6.74 | −0.471 | −3.05 |
|  |  | Something about silencing protein 10 (UTP3) | Q9NQZ2 | 54557.98 | 5.5 | −1.023 | −3.05 |
|  |  | Isoform C1 of Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) | P07910 | 33670.01 | 4.95 | −0.977 | −3.52 |
|  |  | LOC344382 similar to Serine-threonine kinase receptor-associated protein (STRAP) | B0AZV0 | 38438.31 | 4.98 | −0.412 | −3.52 |
|  |  | Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 (CHCHD3) | Q9NX63 | 26152.36 | 8.48 | −1.021 | −4.71 |
| Â | Reduction-oxidation reactions | Â | Â | Â | Â | Â | Â |
|  |  | Superoxide dismutase (Mn), mitochondrial precursor (SOD2) | P04179 | 24722.09 | 8.35 | −0.418 | −3.12 |
| Â | Cell structure and motility | Â | Â | Â | Â | Â | Â |
|  |  | Vimentin (VIM) | P08670 | 53651.68 | 5.06 | −0.823 | −3.53 |
|  |  | Poliovirus receptor-related protein 1 (Precursor) (PVRL1) | Q15223 | 57158.18 | 5.77 | −0.490 | −2.26 |
|  |  | Bullous pemphigoid antigen 1, isoforms 6/9/10 (DSJ) | O94833 | 590992.91 | 5.49 | −0.674 | −3.05 |
|  |  | Centrosomal protein of 170 kDa (CEP170) | A6H8 × 9 | 175292.98 | 6.64 | −0.898 | −3.05 |
|  |  | MYH9 | P35579 | 226532.24 | 5.5 | −0.854 | −2.67 |
|  |  | MYH11 | Q4G140 | 24976.53 | 6.54 | −0.440 | −3.52 |
|  |  | ACTB Actin | P60709 | 41736.73 | 5.29 | −0.200 | −4.44 |
|  |  | ACTA2 Actin | P62736 | 42008.95 | 5.24 | −0.233 | −3.52 |
| Â | Signaling | Â | Â | Â | Â | Â | Â |
|  |  | Probable G-protein coupled receptor 81 (GPR81) | Q9BXC0 | 39295.31 | 9.14 | 0.349 | −3.52 |
| Â | Other | Â | Â | Â | Â | Â | Â |
|  |  | Embigin precursor (EMB) | Q6PCB8 | 36880.95 | 6.13 | −0.393 | −3.05 |
|  |  | B2M β-2-microglobulin (B2M) | P61769 | 13714.57 | 6.06 | −0.376 | −3.05 |
| Â | Unknown | Â | Â | Â | Â | Â | Â |
|  |  | Noelin-2 precursor (OLFM2) | O95897 | 51386.34 | 8.06 | −0.309 | −3.28 |
|  |  | CDNA FLJ41134 fis, clone BRACE2 (Q6ZWG4) | 027970 | 15844.35 | 10.57 | −0.193 | −3.52 |