Figure 2
From: IGF-1 Expression in Infarcted Myocardium and MGF E Peptide Actions in Rat Cardiomyocytes in Vitro
Expression of the IGF-1 isoforms in rat myocardium. Panel I: Specific PCR products for MGF and IGF-1Ea mRNA detected in rat myocardium. The PCR products were confirmed by sequencing. Panel II: Detection of MGF and IGF-1Ea at the protein level using Western blot analysis. Panel III: Photomicrographs (magnification, × 40) of the left ventrical sections of rat myocardium stained with anti-MGF antibody. Specificity of the immunohistochemical MGF detections was confirmed by the absence of immunoreactivity in the negative control sections (A). Note that myocardial tissues of sham-operated rats appear less intensively stained (B) compared with infarcted tissues (C).