Figure 3
From: IGF-1 Expression in Infarcted Myocardium and MGF E Peptide Actions in Rat Cardiomyocytes in Vitro
Panel I: mRNA expression of IGF-1Ea and MGF splice variants at different time points after experimental occlusion of the left descending coronary artery. Bars represent mean values (mean ± SEM of six measurements) of respective mRNA expression, which was normalized to each corresponding ribosomal 18S and expressed as the fold change of the mRNA levels in sham-operated rats (controls). PCR amplification for both 18S and the different IGF-1 transcripts was in linear phase at 37 cycles at all time points. *Statistically significant values: P < 0.05. Panel II: Expression of IGF-1Ea and MGF at the protein level using Western blot analysis. Immunoblotting for GAPDH served as a control for protein loading. Expression values (means ± SEM of six measurements) were normalized to those of corresponding GAPDH in the same immunoblot and expressed as the fold change of the sham-operated rats (controls). *Statistically significant values: P < 0,05.