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Figure 3 | Molecular Medicine

Figure 3

From: A Novel Recombinant Vaccinia Virus Expressing the Human Norepinephrine Transporter Retains Oncolytic Potential and Facilitates Deep-Tissue Imaging

Figure 3

Localization of the hNET protein on the cell membrane of nonpermeabilized PANC-1 cells infected with GLV-1h99. PANC-1 cells were mock-infected or infected with GLV-1h68 or GLV-1h99 at an MOI of 0.001 for 24 h. The hNET protein was detected by immunofluorescence microscopy using monoclonal anti-hNET antibody NET17-1, which recognizes the extracellular domain of the protein. Mock- or GLV-1h68-infected cells (as demonstrated by GFP expression) did not express the hNET protein, whereas the hNET protein on the cell membrane of PANC-1 cells infected with GLV-1h99 was readily detectable.

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Molecular Medicine

ISSN: 1528-3658