Figure 6

Effect of PROG and VDH exposure on the activation of MAPK in primary cortical neurons. Cells were exposed to hormones either separately or in various combinations for 30 min. Cells were lysed after incubation, and lysates were separated on 12.5% SDS gel and transferred onto polyvinylidene difluoride membrane. Membrane was probed with either phospho-ERK1/2 or total ERK1/2 protein. Phospho-ERK1/2 data were normalized with total ERK1/2 protein. Data were analyzed using ANOVA and the Neuman-Keuls test. Values are expressed as SEM of three independent experiments. Significant difference: *P< 0.05 compared with control; ^†P < 0.05 compared with PROG (20 µmol/L) and VDH (20 nmol/L) groups. Values in parentheses represent fold increase in MAPK over control values.