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Table 3 Summary of the single copy (sc) lines generated, transgene copy numbers, γ-globin mRNA expression levels in adult blood, and integration sites of the transgene.

From: Persistent Fetal γ-Globin Expression in Adult Transgenic Mice following Deletion of Two Silencer Elements Located 3′ to the Human Aγ-Globin Gene

Transgenic lines

Copy no.

% γ/βmaj Globina

% γ/βmaj Globin per copya

Chromosomal integration site

LCR-Aγ-ΔEnh/F-1sc

1

5.90 ± 0.26

11.80 ± 0.52

Centromeric

LCR-Aγ-ΔEnh/F-2sc

1

7.97 ± 1.15

15.94 ± 2.30

Euchromatic

LCR-Aγ-ΔEnh/F-3sc

1

8.75 ± 1.64

17.50 ± 3.28

Euchromatic

  1. aExpression of the fetal Aγ-gene was calculated by S1 nuclease analysis as 1) a percentage of total βmaj globin expression and 2) per transgene copy and per copy of the endogenous βmaj mouse globin gene. Mean and standard deviations are derived from three independent RNA samples.
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Molecular Medicine

ISSN: 1528-3658