Persistent Fetal γ-Globin Expression in Adult Transgenic Mice following Deletion of Two Silencer Elements Located 3′ to the Human Aγ-Globin Gene

Table 4 Real-time PCR analysis of γ-globin expression in adult blood of the generated single copy (sc) transgenic lines.

Transgenic lines	% Expression γ-globin/γ-globin of 16.5 dpc embryo normalized to HPRT^a
HPFH2C 16.5 dpc embryo	100
LCRAγ-C	0
LCRAγ-H	0
LCR-Aγ-ΔEnh/F-1sc	18 ± 1
LCR-Aγ-ΔEnh/F-2sc	45 ± 2
LCR-Aγ-ΔEnh/F-3sc	43 ± 5

^aNormalization for the amount of template was performed using primers specific for exon 8 of the mouse HPRT gene. The results shown (mean ± standard deviation) represent the average of at least three independent RNA samples per each line.

ISSN: 1528-3658