Figure 1
Hyperglycemia alters the differentiation potential of myeloid progenitors in BM. (A) Relative change in the number of bone marrow-derived EPC, Mph, and DC from STZ-treated mice (black bars) compared with control mice (white bars). Values for EPC, Mph, and DC derived from BM from mice treated with buffer are set to 100%. Top panel: number of CD31high/Dil-acLDL-positive, attaching cells in EPC cultures (C57BL/6J; 4 experiments, nbuffer = 18 and nSTZ = 26 and FVB/N; 5 experiments, nbuffer = 17 and nSTZ = 23). Middle panel: the number of F4/80-positive cells in M-CSF-stimulated Mph cultures (C57BL/6J; 3 experiments, nbuffer = 13 and nSTZ = 18 and FVB/N; 3 experiments, nbuffer = 13 and nSTZ = 16). Bottom panel: number of CD11c-positive cells in GM-CSF-stimulated dendritic cell cultures (C57BL/6J; 3 experiments, nbuffer = 13 and nSTZ = 18 and FVB/N; 3 experiments, nbuffer = 13 and nSTZ = 16). (B) Correlations between the number of cultured EPC (top panel), Mph (middle panel), and DC (bottom panel) and hyperglycemia as assessed by HbA1c. Representative experiment using BM from hyperglycemic C57BL/6J mice (n = 9).