Figure 2
Hyperglycemia has no major quantitative impact on the myeloid progenitor fractions of the BM. BM of the control and hyperglycemic mice was harvested and immediately stained with anti-CD31 and anti-Ly-6C. Using this combination of markers, we identified 6 distinct populations (P1-P6). The populations consisted of: (P1) 70% blast cells and 25% lymphoid cells, (P2) lymphoid cells, (P3) erythroid cells, (P4) myeloid progenitors and plasmacytoid cells, (P5) granulocytes, (P6) 75% monocytes and 20% monocyte progenitors (26). For each subpopulation, the average increase or decrease in BM cell fractions from hyperglycemic mice is depicted as percentage compared with control mice. In the top panel representative scatter plots from hyperglycemic BM are shown for each strain. For both strains the average percentage and standard deviations were calculated from 3 experiments (C57BL/6J: nbuffer = 15 and nSTZ = 18 and FVB/N; nbuffer = 13 and nSTZ = 14, *P< 0.001).