Differentiation of Bone Marrow-Derived Endothelial Progenitor Cells Is Shifted into a Proinflammatory Phenotype by Hyperglycemia

Table 2 Angiogenic and proinflammatory properties.^a

	C57BL/6J Control (n)	C57BL/6J STZ (n)	P value	FVB/N Control (n)	FVB/N STZ (n)	P value
Angiogenesis length tubes, AU	0.47 ± 0.17 (8)	0.27 ±0.12 (8)	0.03^b	0.77 ±0.19 (12)	0.50 ±0.20 (12)	0.02^b
Endocytosis EPC Δ geo mean	63.6 ± 18.0 (9)	78.8 ±21.2 (15)	0.12	20.7 ± 17.9 (8)	42.3 ± 15.2 (8)	0.09
Endocytosis Mph Δ geo mean	47.0 ± 8.6 (3)	62.7 ± 8.0 (3)	0.13	78.3 ±7.6 (3)	68.8 ± 11.4 (3)	0.28
Mixed lymphocyte reaction Δ 3H-thymidine incorporation (%)	100 (9)	176 ± 71 (15)	0.15	100 (6)	139 ± 27 (8)	0.08
IL-12p40 concentration, pg/mL	26.9 ± 13.2 (8)	64.4 ± 19.3 (8)	0.001^b	29.3 ± 13.5 (8)	56.6 ± 24.3 (8)	0.02^b
IL-12p70 concentration, pg/mL	78.4 ± 28.1 (8)	155 ±52.5 (8)	0.006^b	62.7 ± 44.3 (8)	262 ± 63.1 (8)	< 0.001^b

^aFunctional analyses of EPC and Mph cultured for 7 d from control and hyperglycemic BM. Listed are the capacity of EPC-conditioned media to stimulate in vitro formation of tubular structures by HUVEC (n = 8, each group), the capacity of EPC and Mph to endocytose large dextran molecules labeled with FITC as measured by fluorescence-activated cell-sorting analyses and expressed as the delta geo mean (A), the capacity of EPC to stimulate T-cell proliferation as assessed by allogeneic mixed lymphocyte reaction (determined by measuring ³H-thymidine incorporation and expressed in percentage compared with EPC from controls), and the expression of the production of proinflammatory cytokines IL-12p40 and IL-12 p70 by EPC after lipopolysaccharide stimulation (n = 8 per group). ^bStatistically significant.

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