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Figure 3 | Molecular Medicine

Figure 3

From: The Selective α7 Agonist GTS-21 Attenuates Cytokine Production in Human Whole Blood and Human Monocytes Activated by Ligands for TLR2, TLR3, TLR4, TLR9, and RAGE

Figure 3

GTS-21 attenuates endotoxin-induced TNF levels in isolated human monocytes in vitro at a transcriptional level. (A) Cultured monocytes were incubated with vehicle, GTS-21, or nicotine at the indicated concentrations 15 min prior to stimulation with endotoxin (10 ng/mL). Cytokine levels in supernatants were determined 4 h after addition of endotoxin. Each drug concentration was tested in duplicate in monocytes obtained from six independent donors. (B) Effect of GTS-21 100 µmol/L on TNF mRNA levels in human monocytes stimulated with endotoxin (10 ng/mL) at 2 h. Each condition was tested in duplicate in monocytes obtained from five independent donors. Data shown as mean ± SEM. **P< 0.01 versus control; #P< 0.01 versus GTS-21 100 nmol/L.

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Molecular Medicine

ISSN: 1528-3658