Figure 1

Differentiation of MSCs and fractalkine expression in MSC/RGDFKN. (A) Differentiation of mouse MSCs in vitro. MSCs were cultured in nondifferentiating medium (a); MSCs cultured in adipogenic media for 14 d were stained with Oil Red O (b); MSCs cultured in osteogenic media for 3 wks were stained with Alizarin Red S (c). (B) Western blotting analysis of fractalkine in MSCs transduced with Ad-RGDFKN or Ad-RGDLacZ. Equal amounts of protein (30 µg per lane) were subjected to Western blotting analysis as described in Materials and Methods. The recombinant extracellular domain of mouse fractalkine (rmFKN) (∼90 kDa, 50 ng per lane) as a positive control was also detected by the same antibody.