Figure 2

Spermine-attenuated, sepsis-induced local and systemic inflammatory responses. Balb/C mice were subjected to sepsis by CLP, and administered with control saline (0.2 mL/ mouse) or spermine (10.0 mg/kg, twice a d) beginning at +0.5 h post CLP At 52 h post CLP, levels of various cytokines in the peritoneal fluid (PF) or serum were determined by cytokine antibody array (A,B,D) and ELISA (C,E), respectively. (A) Representative cytokine antibody arrays. Peritoneal lavage fluid was pooled from three normal mice (−CLP), three septic mice receiving saline (+CLP), and three septic mice receiving spermine (+CLP +SPM), and assayed for cytokine levels by antibody arrays. (B) Relative peritoneal cytokine levels. The optical intensities (AU) for each pair of cytokine spots were determined using a Gel & Graph Digitizing Software, and expressed as mean ± SD of three independent experiments (n = 3). ^*P < 0.05 versus saline (+CLP). (C,E) ELISA analysis of IL-6 levels. (D) Relative serum cytokine levels. ^*P < 0.05 versus saline group (+CLP).