Figure 3
From: Spermine Protects Mice Against Lethal Sepsis Partly by Attenuating Surrogate Inflammatory Markers
Spermine-attenuated peritoneal HMGB1 levels. Balb/C mice were subjected to sepsis by CLP, and administered with control saline (0.2 mL/mouse) or spermine (10 mg/kg, twice a d) beginning at + 0.5 h post CLP At 52 h post CLP, levels of HMGB1 in the peritoneal fluid (PF) or serum were determined by Western blotting analysis. (A) Representative Western blots. −CLP, two normal mice; +CLP, two septic mice receiving saline; +CLP +SPM, two septic mice receiving spermine. (B) Relative HMGB1 levels. The relative band intensities were quantified using the NIH image 1.59 software to determine HMGB1 levels with reference to standard curves generated with purified HMGB1. *P< 0.05 versus saline group (+CLP).