Figure 5
From: Spermine Protects Mice Against Lethal Sepsis Partly by Attenuating Surrogate Inflammatory Markers
Spermine-attenuated, HMGB1-induced cytokine release. Primary peritoneal macrophages were stimulated with LPS (100 ng/mL), HMGB1 (1.0 µg/mL), either alone or in the preence of spermine at indicated doses for 16 h, and levels of various cytokines in the culture medium were assayed by ELISA (A,B) or cytokine antibody arrays (C,D). (A,B) ELISA assay of TNF and IL-6 levels. (C,D) Cytokine antibody arrays of various cytokines. The optical intensities (in arbitrary units, AU) for each pair of cytokine spots were expressed as mean ± SD of three independent experiments (n = 3). *P < 0.05 versus LPS or HMGB1 group, respectively.