Table 2 Summary of RT-PCR primer sequences, position, annealing temperature, and chromosome mapping position of the stemness-related target genes.
From: Expression Pattern of Stemness-Related Genes in Human Endometrial and Endometriotic Tissues
Gene | Primer position | Primer sequence | Annealing temperature, °C | PCR product, bp | Chromosome mapping of the gene |
|---|---|---|---|---|---|
GAPDH | 472 | 5′-GCATCCTGCACCACCACCTG-3′ | 55 | 347 | 12p13 |
|  | 799 | 5′-GCCTGGTTCACGACGTTCTT-3′ |  |  |  |
SOX2 | 1563 | 5′-CCATCCACACTCACGCAAAA-3′ | 59 | 139 | 3q27 |
|  | 1701 | 5′-TATACAAGGTCCATTCCCCCG-3′ |  |  |  |
OCT4 | 1121 | 5′-TCCCATGCATTCAAACTGAGG-3′ | 60 | 103 | 6p21,31 |
|  | 1223 | 5′-CCAAAAACCCTGGCACAAACT-3′ |  |  |  |
NANOG | 1169 | 5′-TGGACACTGGCTGAATCCTTC-3′ | 59 | 142 | 12p13,31 |
|  | 1310 | 5′-CGTTGATTAGGCTCCAACCAT-3′ |  |  |  |
KLF4 | 1508 | 5′-CTGCGGCAAAACCTACACAA-3′ | 60 | 182 | 9q31 |
|  | 1689 | 5′-GGTCGCATTTTTGGCACTG-3′ |  |  |  |
ERAS | 969 | 5′-AATGTAGACCTTTCCCCAGGC-3′ | 58 | 135 | Xp11,23 |
|  | 1103 | 5′-AAAGCCCCTCACCAAGTGAA-3′ |  |  |  |
GDF3 | 778 | 5′-AAAAGGAAGAGCAGCCATCCCT-3′ | 60 | 110 | 12p13.1 |
|  | 887 | 5′-GCAATGATCCACTTGTGCCAA-3′ |  |  |  |
SOX15 | 315 | 5′-GAACAGGTTGGAAGCAAAGGC-3′ | 59 | 127 | 17p13 |
|  | 441 | 5′-GCGTCGATCCTGAAAATGGA-3′ |  |  |  |
DPPA2 | 798 | 5′-AGCCATGTTGGCATCATGG-3′ | 58 | 108 | 3q13,13 |
|  | 905 | 5′-GAGGCTTGCAGCAAAAAGGC-3′ |  |  |  |
SALL4 | 2394 | 5′-GCCCAGATATCCTGGAAACCA-3′ | 60 | 115 | 20q13,13/13,2 |
|  | 250 | 5′-TTCTCGGAGCTCTCTGCTTTG-3′ |  |  |  |
TCL1 | 667 | 5′-CTCGGCTTTTTCTCAGCTGGAT-3′ | 59 | 127 | 14q32,1 |
|  | 793 | 5′-GGTGAATCGGCTGTGTTCTCA-3′ |  |  |  |
ZFP42 | 953 | 5′-ATGACAGTCTGAGCGCAATCG-3′ | 60 | 133 | 4q35,2 |
|  | 1085 | 5′-AACGCTTTCCCACATTCCG-3′ |  |  |  |
UTF1 | 876 | 5′-CGACATCGCGAACATCCTG-3′ | 64 | 117 | 10q26 |
|  | 992 | 5′-AGAATGAAGCCCACGGCCA-3′ |  |  |  |
BMI1 | 437 | 5′-AATGTCTTTTCCGCCCGCT-3′ | 59 | 139 | 10p11,23 |
|  | 575 | 5′-ACCCTCCACAAAGCACACACAT-3′ |  |  |  |