Figure 2
From: Platelet Factor 4 Is Highly Upregulated in Dendritic Cells after Severe Trauma
Realtime RT-PCR for PF4; median and interquartile ranges are shown. Results are presented as fold change of PF4 gene expression on the d of admission (d 0). Messenger RNA was isolated from highly purified DCs obtained from whole blood of multiple trauma patients on d 0, 1, and 4 after admission. Gene expression was determined by the comparative 2-ΔΔCt method as described in the Materials and Methods section. A significant increase of PF4 expression on d 4 compared with d 0 was found (*P < 0.05).