Figure 3
From: The Role of Macrophage Migration Inhibitory Factor in Alzheimer’s Disease
The MIF specific small molecule inhibitor ISO-1 counter-regulates the neurotoxic effect of Aβ1–40, at concentrations of 5 µmol/L or 10 µmol/L in murine BV2 microglial cells. At a concentration of 5 µmol/L, Aβ viability of the cells was reduced to 73%, ISO-1 at 50 or 100 µmol/L restored the Aβ dependent toxicity to 85% of the control. This effect was even more prominent when the cells were treated with 10 µmol/L of Aβ. Here, we obtained a toxic Aβ effect of around 60% in comparison to the control cells. That effect was reduced with 50 µmol/L ISO-1 to around 80% and further reduced to around 90% with 100 µmol/L of ISO-1. The graph displays the measurements of the mean from three separate experiments, performed in quadruplicate. The results did not reach statistical significance and can therefore only be interpreted as a trend.