Figure 2
From: Integration of HIV-1 DNA Is Regulated by Interplay between Viral Rev and Cellular LEDGF/p75 Proteins
Rev promotes dissociation of the IN-LEDGF/p75 complex while the LEDGF/p75 fails to promote dissociation of the Rev-IN complex. (A) Rev-GFP conjugate (
) and Tat (
) were added to plate-bound IN-LEDGF/p75 complex and the amount of bound LEDGF/p75 was estimated. (B) LEDGF/p75 (
) and Tat (
) were added to plate-bound Rev(-GFP)-IN complex and the amount of bound Rev-GFP was estimated. (C) Same as in B but the amount of bound LEDGF/p75 and Tat was estimated. (D) Rev-GFP (
), Rev-GFP and LEDGF/p75 (
) or Rev-GFP and Tat (
) were added to plate-bound IN and the amount of bound Rev-GFP was estimated. (E) LEDGF/p75 (
), Rev-GFP and LEDGF/p75 (
) or LEDGF/p75 and Tat (
) were added to plate-bound IN and the amount of bound LEDGF/p75 was estimated. (F) Apparent Kd as calculated from the changes in affinity presented in (D) and (E). All other details are described in Materials and Methods.