Figure 1

UCB induces neuronal nNOS expression in neurons, which is counteracted by l-NAME. Primary cultures of rat neurons were treated for 4 h at 37°C with either 100 µmol/L UCB, 100 µmol/L l-NAME, UCB plus l-NAME or no addition (control), in the presence of 100 µmol/L HSA. The expression of nNOS was evaluated by Western blotting, as described in Materials and Methods. (A) Blot representative of at least four separate experiments. (B) The intensity of the bands was quantified by scanning densitometry, normalized with respect to endogenous β-actin and expressed as fold change compared with control. **P< 0.01 from control; ^$$P< 0.01 from UCB alone.