Figure 2

UCB induces nitrite formation in neurons, which is abrogated by l-NAME. Primary cultures of rat neurons were treated for 4 h at 37°C with 100 µmol/L UCB, 100 µmol/L l-NAME, UCB plus l-NAME or no addition (control), in the presence of 100 µmol/L HSA. The formation of NO· was indirectly evaluated by measuring nitrites in the incubation medium using the Griess reagent, as described in Materials and Methods. **P< 0.01 from control; ^$$P< 0.01 from UCB alone.