Figure 4

UCB induces protein oxidation in neurons, which is prevented by l-NAME. Primary cultures of rat neurons were treated for 4 h at 37°C with either 100 µmol/L UCB, 100 µmol/L l-NAME, UCB plus l-NAME or no addition (control), in the presence of 100 µmol/L HSA. Protein oxidation was assessed by quantification of carbonyls by slot blot analysis, as described in Materials and Methods. **P< 0.01 from control; ^$P< 0.05 from UCB alone.