Figure 5

UCB impairs GSH homeostasis in neurons, which is restored by l-NAME. Primary cultures of rat neurons were treated for 4 h at 37°C with 100 µmol/L UCB, 100 µmol/L l-NAME, UCB plus l-NAME or no addition (control), in the presence of 100 µmol/L HSA. Intracellular levels of reduced GSH were determined by an enzymatic recycling procedure, as described in Materials and Methods. *P< 0.05 from control; ^$P< 0.05 from UCB alone.